The BioPlex (biophysical interactions of ORFeome-based complexes) network is the result of creating thousands of cell lines with each expressing a tagged version of a protein from the ORFeome collection. Immunopurification of the tagged protein and detection of associated proteins by mass spectrometry are the building blocks of the network. The overarching project goal is to determine protein interactions for every member of the collection. A first paper in Cell reports the first ~2,500 experiments (~23,000 interactions). More recently, an updated version of the network (BioPlex 2.0) was published in Nature resulting from 5891 AP-MS experiments that doubles the span of the resulting network to over 50,000 interactions among ~11,000 proteins. Our latest unpublished version of the BioPlex network incorporates 7500 independent AP-MS experiments to create a network of more than 70,000 interactions.

Department of Cell Biology

A Gygi & Harper Lab Collaboration


  • Biogen
  • NHGRI: U41 HG006673

Clone Availability:

All lentiviral expression constructs used in this study are available at the DNA Resource Core.

Primary BioPlex References:

BioPlex 1.0

Huttlin EL, Ting L, Bruckner RJ, Gebreab F, Gygi MP, Szpyt J, Tam S, Zarraga G, Colby G, Baltier K, Dong R, Guarani V, Vaites LP, Ordureau A, Rad R, Erickson BK, Wühr M, Chick J, Zhai B, Kolippakkam D, Mintseris J, Obar RA, Harris T, Artavanis-Tsakonas S, Sowa ME, De Camilli P, Paulo JA, Harper JW, Gygi SP. (2015) The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 162:425-440.

BioPlex 2.0

Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW. (2017) Architecture of the Human Interactome Defines Protein Communities and Disease Networks. Nature 545:505-509.

Other BioPlex References:

BioPlex Viewer

Schweppe DK, Huttlin EL, Harper JW, Gygi SP. (2017) BioPlex Display: An Interactive Suite for Large-scale, AP-MS Protein-protein Interaction Data. J. Proteome Res. doi: 10.1021/acs.jproteome.7b00572.